BimS, EL, L (AA 20-40) antibody

Catalog No. ABIN2745516

The Rat Monoclonal anti- antibody has been validated for FACS, IP, IHC, WB, ICC and ELISA. It is suitable to detect in samples from Mouse, Dog, Human, Monkey and Rat.

Quick Overview for BimS, EL, L (AA 20-40) antibody (ABIN2745516)

Target: BimS, EL, L

Reactivity: Mouse, Dog, Human, Monkey, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), ELISA

Clone: 3C5

Product Details

Binding Specificity: AA 20-40

Specificity: Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19 kDa), BimEL (~23 kDa), and BimS (~16 kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region.

Cross-Reactivity: Dog (Canine), Human, Monkey, Mouse (Murine), Rat (Rattus)

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Synthetic peptide corresponding to aa 20-40 of mouse BimL.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide and 25 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details

Target: BimS, EL, L

Alternative Name: BimS/EL/L

Background: Bim belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the Bcl-2 protein family (BCL2, BCL2L1/BCL-X(L) and MCL1) and to act as an apoptotic activator, inducing apoptosis and anoikis. Several variants have been identified. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.